7P46
Crystal Structure of Xanthomonas campestris Tryptophan 2,3-dioxygenase (TDO)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-02-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97828 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.095, 117.752, 138.819 |
| Unit cell angles | 90.00, 95.52, 90.00 |
Refinement procedure
| Resolution | 89.780 - 1.700 |
| R-factor | 0.1507 |
| Rwork | 0.149 |
| R-free | 0.18510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nw8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.692 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 117.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.055 | 2.900 |
| Number of reflections | 268120 | 34391 |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 98.0 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 1000, MES (2-(N-morpholino)ethanesulfonic acid) pH 6.3, bicine (N,N-bis(2-hydroxyethyl)glycine) pH 9.0, MnCl2, MgCl2, sodium cyanide and L-Trp |
