7P3A
N-terminal domain of CGI-99
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-14 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0084 |
| Spacegroup name | P 61 |
| Unit cell lengths | 91.714, 91.714, 52.942 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.860 - 2.000 |
| R-factor | 0.1979 |
| Rwork | 0.197 |
| R-free | 0.22420 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.105 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (1.16_3549) |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.860 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.142 | 1.530 |
| Rpim | 0.032 | 0.354 |
| Number of reflections | 17246 | 3450 |
| <I/σ(I)> | 18.6 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 20.2 | 19.3 |
| CC(1/2) | 0.999 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100 mM HEPES pH 7.5, 200 mM magnesium chloride, 20% isopropanol |
