7P2J

Dimethylated fusion protein of RSL and trimeric coiled coil (4dzn) in complex with cucurbit[7]uril, H3 sheet assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98
Spacegroup name	H 3
Unit cell lengths	51.617, 51.617, 322.633
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	107.540 - 1.980
R-factor	0.2033
Rwork	0.201
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bt9
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.540	2.010
High resolution limit [Å]	1.980	1.980
Rmeas	0.170	0.916
Rpim	0.055	0.334
Number of reflections	22317	1086
<I/σ(I)>	6.8	2.1
Completeness [%]	100.0	100
Redundancy	8.6	7.3
CC(1/2)	0.990	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20 % PEG 8000 0.1 M Tris-HCl pH 8.5 0.2 M MgCl2 2 mM Q7