7P2I

Dimethylated fusion protein of RSL and nucleoporin peptide (Nup) in complex with cucurbit[7]uril, F432 cage assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98
Spacegroup name	F 4 3 2
Unit cell lengths	200.713, 200.713, 200.713
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.360 - 1.489
R-factor	0.1624
Rwork	0.162
R-free	0.17790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bt9
RMSD bond length	0.007
RMSD bond angle	0.770
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BUSTER
Refinement software	BUSTER (2.10.3 (23-SEP-2019))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.360	1.520
High resolution limit [Å]	1.489	1.489
Rmeas	0.012
Rpim	1.400
Number of reflections	56936	2792
<I/σ(I)>	28.7	2.2
Completeness [%]	100.0	100
Redundancy	7.3	6.9
CC(1/2)	1.000	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25 % PEG 3350 0.1 M Bis-Tris pH 5.5 0.2 M MgCl2