7P26
Sulfated host glycan recognition by carbohydrate sulfatases of the human gut microbiota (BT4631_S1_15)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 49.924, 109.572, 117.531 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.274 - 1.350 |
| Rwork | 0.123 |
| R-free | 0.13910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7oze |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.482 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.570 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 140145 | 6050 |
| <I/σ(I)> | 25.4 | 4.5 |
| Completeness [%] | 98.8 | |
| Redundancy | 12.4 | |
| CC(1/2) | 0.999 | 0.955 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 80 mg/ml BT4631 with 10 mM 6S-Gal was crystallised in 20 % PEG 10K and 0.1 M Bicine pH8.5. |
