7P1V
Apo structure of KDNase from Trichophyton Rubrum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 173 |
Detector technology | PIXEL |
Collection date | 2019-07-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9688 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.030, 180.850, 98.340 |
Unit cell angles | 90.00, 103.77, 90.00 |
Refinement procedure
Resolution | 47.360 - 1.470 |
R-factor | 0.1767 |
Rwork | 0.174 |
R-free | 0.22180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xcy |
RMSD bond length | 0.010 |
RMSD bond angle | 1.350 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.400 | 1.510 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmerge | 0.066 | 0.775 |
Number of reflections | 276720 | 20438 |
<I/σ(I)> | 7.3 | |
Completeness [%] | 98.5 | |
Redundancy | 3.2 | |
CC(1/2) | 0.990 | 0.400 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Calcium chloride dihydrate 0.1 M MES 6.0 20 % w/v PEG 6000 |