7P0V

Crystal structure of human SF3A1 ubiquitin-like domain in complex with U1 snRNA stem-loop 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999987
Spacegroup name	I 2 2 2
Unit cell lengths	42.993, 62.722, 138.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.050 - 1.560
R-factor	0.2198
Rwork	0.218
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zkh
RMSD bond length	0.007
RMSD bond angle	0.861
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.050	1.620
High resolution limit [Å]	1.560	1.560
Number of reflections	26828	4256
<I/σ(I)>	21.73
Completeness [%]	99.0
Redundancy	13.3
CC(1/2)	1.000	0.555

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	100 mM Tris-HCl pH 8.5, 200 mM Li2SO4 and 40 % PEG400