7P0A

CRYSTAL STRUCTURE OF THE MURINE CLASS I MAJOR HISTOCOMPATIBILITY COMPLEX H-2DB IN COMPLEX WITH LCMV-DERIVED GP33 PEPTIDE with D-AMINOACID (p3P6f)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	120.649, 125.958, 92.927
Unit cell angles	90.00, 126.63, 90.00

Refinement procedure

Resolution	48.409 - 2.429
R-factor	0.2134
Rwork	0.210
R-free	0.25640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nsk
RMSD bond length	0.003
RMSD bond angle	0.678
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.409	2.680
High resolution limit [Å]	2.429	2.430
Number of reflections	30282	30282
<I/σ(I)>	2
Completeness [%]	93.2
Redundancy	6.5
CC(1/2)	0.994	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	1.7 M Ammonium Sulphate, 100 mM Tris-HCl, pH 8.3