7P01

Structure of the maltase BaAG2 from Blastobotrys adeninivorans in complex with acarbose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-29
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979965
Spacegroup name	P 1 21 1
Unit cell lengths	68.030, 78.080, 121.930
Unit cell angles	90.00, 94.10, 90.00

Refinement procedure

Resolution	46.320 - 2.120
R-factor	0.1846
Rwork	0.184
R-free	0.19520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3aj7
RMSD bond length	0.019
RMSD bond angle	1.888
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.320	2.180
High resolution limit [Å]	2.120	2.120
Number of reflections	140413	10323
<I/σ(I)>	7.68	1.54
Completeness [%]	98.9	98.7
Redundancy	3.6	3.6
CC(1/2)	0.970	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.1 M MES pH 5.5 12% polyvinylpyrrolidone