7OYW

E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D F88L S247D in complex with spermidine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 2
Unit cell lengths	116.881, 71.285, 92.417
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.390 - 1.280
R-factor	0.1327
Rwork	0.132
R-free	0.16130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a99
RMSD bond length	0.008
RMSD bond angle	1.045
Data reduction software	XDS (20190806)
Data scaling software	XSCALE (20190806)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.390	1.330
High resolution limit [Å]	1.280	1.280
Rmerge	0.104	2.316
Rmeas	0.114	2.536
Rpim	0.044	1.015
Number of reflections	198363	19073
<I/σ(I)>	8.8	0.59
Completeness [%]	99.3	96.4
Redundancy	6.6	6
CC(1/2)	0.997	0.233

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	2.4 M AmSO4 + 0.1 M Bicine pH 9.0 + 5% Jeffamine M600