7OXW
CrabP2 mutant R30DK31D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 45.300, 92.290, 98.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.145 - 1.160 |
| Rwork | 0.162 |
| R-free | 0.17370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2frs |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.720 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.150 | 1.180 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Number of reflections | 71395 | 3221 |
| <I/σ(I)> | 19.4 | 2.5 |
| Completeness [%] | 99.4 | 91.8 |
| Redundancy | 6.4 | 2.8 |
| CC(1/2) | 0.999 | 0.946 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 100 mM Sodium acetate pH 4.5 and 2.0 M ammonium sulfate |
