7OXS

1.91 A crystal structure of DNA/2'-O-methyl-RNA heteroduplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-02
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.91840
Spacegroup name	P 32 2 1
Unit cell lengths	56.130, 56.130, 76.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.420 - 1.910
R-factor	0.1848
Rwork	0.184
R-free	0.19540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ow0
RMSD bond length	0.008
RMSD bond angle	1.109
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.610	2.020
High resolution limit [Å]	1.910	1.910
Rmeas	0.033	1.063
Number of reflections	20931	3348
<I/σ(I)>	31.56
Completeness [%]	99.6	97.9
Redundancy	10.3	2.01
CC(1/2)	1.000	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2.0 M Li2SO4, 50 mM sodium cacodylate pH 6.5, 10 mM spermine, 15 mM MgCl2