7OXQ
Crystal structure of HIV-1 reverse transcriptase with a double stranded DNA in complex with fragment 048 at the transient P-pocket.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91589 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 309.565, 61.900, 168.779 |
| Unit cell angles | 90.00, 104.55, 90.00 |
Refinement procedure
| Resolution | 81.680 - 3.300 |
| R-factor | 0.2634 |
| Rwork | 0.262 |
| R-free | 0.29640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6amo |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.750 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.323 | 0.953 |
| Rmeas | 0.377 | 1.104 |
| Rpim | 0.189 | 0.544 |
| Number of reflections | 44516 | 4374 |
| <I/σ(I)> | 3.3 | 1.4 |
| Completeness [%] | 94.6 | 95.3 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.973 | 0.251 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 11-12% v/v PEG Smear Broad, 10% w/v sucrose, 50 mM PIPES-NaOH pH 6.5, 0.1 M (NH4)2SO4, 5 mM MgCl2, 5 mM CdCl2 |
