7OXD
Crystal structure of Scytonema hofmanni transposition protein TniQ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-16 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.636, 49.875, 86.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.240 - 1.300 |
| R-factor | 0.1889 |
| Rwork | 0.188 |
| R-free | 0.19520 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.400 | 1.346 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.046 | 0.964 |
| Rmeas | 0.047 | |
| Rpim | 0.013 | 0.285 |
| Number of reflections | 85839 | 53615 |
| <I/σ(I)> | 32.11 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.7 | 12.1 |
| CC(1/2) | 1.000 | 0.889 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 280 | 100 mM HEPES pH 7.5 20% PEG 6000 250 mM sodium chloride |
