7OXA

Target-bound SpCas9 complex with AAVS1 chimeric RNA-DNA guide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-05
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	177.926, 67.574, 189.232
Unit cell angles	90.00, 112.52, 90.00

Refinement procedure

Resolution	47.660 - 2.150
R-factor	0.1991
Rwork	0.198
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fq5
RMSD bond length	0.004
RMSD bond angle	0.618
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.660	2.227
High resolution limit [Å]	2.150	2.150
Rmerge	0.113	2.230
Number of reflections	113319	11255
<I/σ(I)>	12.36	0.78
Completeness [%]	99.9	99.81
Redundancy	6.8	6.7
CC(1/2)	0.999	0.282

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	0.1 M Tris-acetate pH 8.5, 0.3-0.5 M KSCN, 17-19% PEG3350