7OX7
Target-bound SpCas9 complex with TRAC chimeric RNA-DNA guide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-08 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.542, 67.406, 188.136 |
| Unit cell angles | 90.00, 112.54, 90.00 |
Refinement procedure
| Resolution | 42.460 - 2.600 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fq5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.622 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.460 | 2.693 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.219 | 2.461 |
| Number of reflections | 63691 | 6337 |
| <I/σ(I)> | 8.54 | 0.74 |
| Completeness [%] | 99.9 | 99.83 |
| Redundancy | 6.8 | 6.8 |
| CC(1/2) | 0.995 | 0.284 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 0.1 M Tris-acetate pH 8.5, 0.3-0.5 M KSCN, 17-19% PEG3350 |
