7OWL

Odinarchaeota Adenylate kinase (OdinAK) in complex with CTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9800
Spacegroup name	P 21 21 21
Unit cell lengths	76.486, 77.631, 216.881
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.010 - 2.900
R-factor	0.1947
Rwork	0.192
R-free	0.25460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7owe
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.010	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.315	1.732
Rpim	0.163	0.904
Number of reflections	29184	2878
<I/σ(I)>	5.5	2.1
Completeness [%]	99.3	100
Redundancy	8.9
CC(1/2)	0.986	0.628

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Protein buffer: 0.030 M MOPS, 0.050 M NaCl pH 7.0. Crystallization: Bis-Tris Propane pH 7.0, 0.1 M, PEG 3350 12-18% Odin 0.678 milliM, CTP 6.37 milliM