7OWK

Odinarchaeota Adenylate kinase (OdinAK) in complex with dTTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9800
Spacegroup name	P 21 21 21
Unit cell lengths	76.824, 77.203, 214.745
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.567 - 3.100
R-factor	0.208
Rwork	0.206
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7owe
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.570	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.356	3.017
Number of reflections	23925	2363
<I/σ(I)>	9.1	2.3
Completeness [%]	100.0	100
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	Protein: 0.030 M MOPS, 0.050 M NaCl pH 7.0. Precipitant: Bis-Tris Propane pH 7.0, 0.1 M, PEG 3350 15-18% Odin 0.732 milliM, dTTP 5.8 milliM