7OW0

1.55 A crystal structure of DNA/2'-O-methyl-RNA heteroduplex with overhangs solved by Zn-SAD.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-07
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.28308
Spacegroup name	P 43 21 2
Unit cell lengths	31.850, 31.850, 91.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.090 - 1.550
R-factor	0.2241
Rwork	0.221
R-free	0.25080
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.624
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXD
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.850	1.640
High resolution limit [Å]	1.550	1.550
Rmerge	0.049	0.586
Rmeas	0.053	0.696
Number of reflections	12966	1979
<I/σ(I)>	18.92	1.96
Completeness [%]	99.0	94.6
Redundancy	6.03	3.36
CC(1/2)	0.998	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1.8 M Li2SO4, 50 mM Na cacodylate pH 6.5, 5 mM spermine, 50 mM ZnCl2