7OVO

Heterodimeric murine tRNA-guanine transglycosylase in complex with queuine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.918400
Spacegroup name	P 43 2 2
Unit cell lengths	100.429, 100.429, 202.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.740 - 2.100
R-factor	0.1912
Rwork	0.189
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h62 6fv5
RMSD bond length	0.007
RMSD bond angle	0.790
Data reduction software	XDS (1.02)
Data scaling software	XDS (1.02)
Phasing software	PHASER (7.0.047)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.100	2.100
Number of reflections	61375	9724
<I/σ(I)>	20.7
Completeness [%]	99.9
Redundancy	13.1
CC(1/2)		0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	100 mM Citrate pH 6.5, 700 mM Ammonium sulfate, 1 M Lithium sulfate