7OUQ

wilavidin biotin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976252
Spacegroup name	I 21 3
Unit cell lengths	180.132, 180.132, 180.132
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.030 - 2.630
R-factor	0.1794
Rwork	0.178
R-free	0.20020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3szj
RMSD bond length	0.011
RMSD bond angle	2.073
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.030	2.770
High resolution limit [Å]	2.630	2.630
Number of reflections	28877	2810
<I/σ(I)>	17.2
Completeness [%]	99.8
Redundancy	21.6
CC(1/2)	0.999	0.392

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.8 M Ammonium sulfate