7OS6

Crystal structure of Rhizobium etli inducible L-asparaginase ReAV (monoclinic form MP1)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	77.812, 91.044, 113.662
Unit cell angles	90.00, 97.08, 90.00

Refinement procedure

Resolution	77.220 - 1.430
Rwork	0.159
R-free	0.17900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	XXXX
RMSD bond length	0.011
RMSD bond angle	1.681
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.220	1.520
High resolution limit [Å]	1.430	1.430
Rmerge	0.073	0.818
Rmeas	0.084	0.952
Number of reflections	287575	45258
<I/σ(I)>	10.2	1.5
Completeness [%]	99.3	96.8
Redundancy	4.1	3.4
CC(1/2)	0.998	0.671

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	292	25% PEG3350, 0.2 M Li2SO4, 0.1 M Tris pH 8.0, 20 mM n-octyl-beta-D-thioglucoside