7ORD
The crystal structure of the domain-swapped dimer of onconase (2)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.750, 28.540, 61.480 |
| Unit cell angles | 90.00, 94.24, 90.00 |
Refinement procedure
| Resolution | 58.590 - 2.140 |
| Rwork | 0.212 |
| R-free | 0.26680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1onc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.636 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.590 | 2.180 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmeas | 0.124 | |
| Number of reflections | 11313 | 11313 |
| <I/σ(I)> | 10.6 | 2.3 |
| Completeness [%] | 97.4 | |
| Redundancy | 5.9 | 4.3 |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris-HCl pH 8.5 25% PEG 3350 0.2 M Ammonium acetate |
