7OQV

Crystal structure of the polymerising VEL domain of VIN3 (I575D mutant)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	31.498, 91.273, 98.413
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.070 - 2.400
Rwork	0.223
R-free	0.26950
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.532
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.070	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	11740	11740
<I/σ(I)>	13.3
Completeness [%]	100.0
Redundancy	8.7
CC(1/2)	1.000	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	1M potassium phosphate monobasic, 3 % v/v 2 propanol, 0.1M sodium cacodylate pH6.5