7OOI

Anti-EphA1 JD1 VH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-15
Detector	RAYONIX MX-300
Wavelength(s)	0.979490
Spacegroup name	P 43 21 2
Unit cell lengths	60.210, 60.210, 168.071
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.580 - 2.280
Rwork	0.269
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b9v
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (v1.14)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.580	6.380	2.280
High resolution limit [Å]	2.150	6.380	2.150
Rmerge	0.260	0.068	2.284
Rmeas	0.270	0.071	2.377
Number of reflections	17674	785	2751
<I/σ(I)>	10.62	27.09	2.01
Completeness [%]	99.9	99.7	99.7
Redundancy	13.552	12.005	12.716
CC(1/2)	0.997	0.999	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	0.2 M ammonium sulphate, 10% glycerol, 0.1 M phosphate citrate (pH 4.2) and 20% (w/v) polyethyleneglycol (PEG) 300