7ON3

SaFtsZ complexed with GDP (soak 10 mM EGTA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9787
Spacegroup name	C 1 2 1
Unit cell lengths	71.858, 50.954, 88.488
Unit cell angles	90.00, 110.98, 90.00

Refinement procedure

Resolution	41.310 - 2.320
R-factor	0.2367
Rwork	0.234
R-free	0.28820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rvn
RMSD bond length	0.001
RMSD bond angle	0.430
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.310	2.400
High resolution limit [Å]	2.320	2.320
Number of reflections	12638	1114
<I/σ(I)>	6.94	1.77
Completeness [%]	96.5
Redundancy	3.1
CC(1/2)	0.986	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2 M lithium sulphate, 10% (v/v) ethylene glycol, 0.1 M Tris-HCl pH 8.5, and 26% polyethylene glycol 5000 monomethyl ether (+ 10 mM EGTA for soaking, 23 h)