7OMG

Crystal structure of KOD DNA Polymerase in a ternary complex with an Uracil containing template

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000009
Spacegroup name	P 21 21 2
Unit cell lengths	108.403, 147.138, 71.129
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.250 - 2.100
R-factor	0.2116
Rwork	0.210
R-free	0.24780
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5omf
RMSD bond length	0.009
RMSD bond angle	1.024
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4085)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.026	2.230
High resolution limit [Å]	2.100	2.100
Rmeas	0.142	0.427
Number of reflections	66516	10239
<I/σ(I)>	11.49	0.39
Completeness [%]	99.2
Redundancy	9.8
CC(1/2)	0.999	0.157

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	Morpheus A3: 0.06MDivalents (0.3M Magnesium chloride hexahydrate; 0.3M Calcium chloride dihydrate) 0.1M Buffer System 1 (1 M MES/Imidazol pH 6.5) 30% v/vPrecipitant Mix 3 (40% v/v Glycerol; 20% w/v PEG 4000)