7OMA

Thosea asigna virus RdRP domain elongation complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	67.794, 207.305, 115.980
Unit cell angles	90.00, 91.10, 90.00

Refinement procedure

Resolution	67.780 - 3.100
R-factor	0.2322
Rwork	0.231
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xhi
RMSD bond length	0.006
RMSD bond angle	0.858
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.780	3.211
High resolution limit [Å]	3.100	3.100
Rmerge	0.384	2.066
Rpim		0.842
Number of reflections	57906	5770
<I/σ(I)>	6.79	1.22
Completeness [%]	99.9	99.84
Redundancy	6.9
CC(1/2)	0.951	0.391

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M sodium acetate pH4.6, 0.22M (NH4)SO4, 28% PEG2000MME