7OM7

Thosea asigna virus RdRP domain in complex with RNA and nucleotide UMPNPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97920
Spacegroup name	P 1 21 1
Unit cell lengths	67.480, 206.266, 115.590
Unit cell angles	90.00, 90.75, 90.00

Refinement procedure

Resolution	59.090 - 2.400
R-factor	0.2147
Rwork	0.215
R-free	0.21550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xhi
RMSD bond length	0.010
RMSD bond angle	1.392
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.090	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.131	1.717
Rmeas	0.136	1.792
Rpim	0.039	0.510
Number of reflections	122219	12216
<I/σ(I)>	17.25
Completeness [%]	99.4	99.58
Redundancy	12
CC(1/2)	0.998	0.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M sodium acetate pH4.6, 0.22M (NH4)SO4, 28% PEG2000MME