7OLZ

Crystal structure of the SARS-CoV-2 RBD with neutralizing-VHHs Re5D06 and Re9F06

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-24
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	54.955, 57.017, 144.029
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.010 - 1.750
R-factor	0.1775
Rwork	0.176
R-free	0.20920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yz5
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.010	1.813
High resolution limit [Å]	1.750	1.750
Rmerge	0.090	1.530
Rmeas	0.094	1.590
Rpim	0.026	0.430
Number of reflections	45701	2363
<I/σ(I)>	17.91	1.99
Completeness [%]	85.0	51.39
Redundancy	13.3	13
CC(1/2)	0.999	0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M MOPS pH 7.5, 2.07 M ammonium sulfate, 0.1 M NDSB-256