7OL9

Crystal structure of C-terminally truncated Bacillus subtilis nucleoid occlusion protein (Noc) complexed to the Noc-binding site (NBS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-21
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	70.501, 99.326, 99.416
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.360 - 2.900
R-factor	0.2322
Rwork	0.230
R-free	0.27750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6y93
RMSD bond length	0.003
RMSD bond angle	1.183
Data reduction software	DIALS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.360	70.360	3.070
High resolution limit [Å]	2.890	8.670	2.890
Rmerge	0.113	0.037	2.693
Rmeas	0.118	0.039	2.797
Rpim	0.032	0.012	0.752
Number of reflections	16242	686	2578
<I/σ(I)>	11.6
Completeness [%]	100.0	100	100
Redundancy	13.1	11.1	13.7
CC(1/2)	0.999	1.000	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	Null