7OL2
Crystal structure of mouse contactin 1 immunoglobulin domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-31 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.959, 134.162, 181.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 107.940 - 3.890 |
R-factor | 0.2223 |
Rwork | 0.220 |
R-free | 0.26950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2om5 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 108.090 | 4.270 |
High resolution limit [Å] | 3.890 | 3.890 |
Number of reflections | 20221 | 3909 |
<I/σ(I)> | 4.4 | 1.5 |
Completeness [%] | 93.9 | |
Redundancy | 9.3 | |
CC(1/2) | 0.961 | 0.524 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 277.15 | 20% w/v PEG 3350 and 0.2 M Magnesium nitrate hexahydrate pH 5.9 |