7OK6
Crystal structure of UNC119B in complex with LCK peptide
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.91589 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.083, 67.004, 106.691 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.742 - 1.949 |
| Rwork | 0.181 |
| R-free | 0.22390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h6a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.594 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.742 | 2.000 |
| High resolution limit [Å] | 1.949 | 1.949 |
| Number of reflections | 29562 | 2145 |
| <I/σ(I)> | 12.7 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.998 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 30 % w/v PEG 4,000, 0.1 M HEPES pH 7.5, 0.2 M CaCl2 |
