7OJE

Crystal structure of the covalent complex between Tribolium castaneum deubiquitinase ZUP and Ubiquitin-PA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-19
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000021
Spacegroup name	P 61
Unit cell lengths	151.607, 151.607, 83.751
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.670 - 2.050
R-factor	0.1862
Rwork	0.185
R-free	0.21860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ei1
RMSD bond length	0.006
RMSD bond angle	0.787
Data reduction software	XDS (20180126)
Data scaling software	XDS (20180126)
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.670	2.123
High resolution limit [Å]	2.050	2.050
Number of reflections	67359	6329
<I/σ(I)>	12.83
Completeness [%]	97.9
Redundancy	8.6
CC(1/2)	0.998	0.411

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	8 % (v/v) Tacsimate pH 7; 22 %PEG3350