7OJA

SaFtsZ(D210N) complexed with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	67.000, 53.194, 84.078
Unit cell angles	90.00, 105.75, 90.00

Refinement procedure

Resolution	41.030 - 2.220
R-factor	0.1856
Rwork	0.183
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rvn
RMSD bond length	0.003
RMSD bond angle	0.612
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.030	2.300
High resolution limit [Å]	2.220	2.220
Number of reflections	14229	1375
<I/σ(I)>	9.47	1.77
Completeness [%]	99.5
Redundancy	3.4
CC(1/2)	0.996	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2 M lithium sulphate, 10% (v/v) ethylene glycol, 0.1 M Tris-HCl pH 8.6, and 26% polyethylene glycol 5000 monomethyl ether