7OI5
Crystal structure of AP2 Mu2 - FCHO2 chimera (GST cleaved)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91589 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.046, 55.380, 167.735 |
| Unit cell angles | 90.00, 109.05, 90.00 |
Refinement procedure
| Resolution | 79.270 - 2.610 |
| R-factor | 0.2414 |
| Rwork | 0.238 |
| R-free | 0.29720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Phaser |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.728 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.270 | 2.650 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Rmerge | 0.163 | 1.576 |
| Rmeas | 0.177 | 1.725 |
| Rpim | 0.068 | 0.693 |
| Number of reflections | 31089 | 1543 |
| <I/σ(I)> | 6.6 | 1 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.995 | 0.374 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.1 | 289 | 20% w/v PEG 3350, 0.2 M Sodium phosphate dibasic dehydrate pH 9.1. The crystal was cryo-protected by soaking in mother liquor supplemented with 25% glycerol. |
