7OHN

SaFtsZ complexed with GDP, AlF4- and Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9787
Spacegroup name	C 1 2 1
Unit cell lengths	70.141, 52.006, 87.492
Unit cell angles	90.00, 109.79, 90.00

Refinement procedure

Resolution	41.160 - 1.620
R-factor	0.1619
Rwork	0.160
R-free	0.20140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rvn
RMSD bond length	0.010
RMSD bond angle	1.099
Data reduction software	XDS (1.18.2_3874)
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.160	1.680
High resolution limit [Å]	1.620	1.620
Number of reflections	37431	3590
<I/σ(I)>	11.9
Completeness [%]	99.2
Redundancy	3.3
CC(1/2)	0.996	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris-HCl pH 8.6, 24% PEG 5000 MME