7OHL

SaFtsZ complexed with GDP, BeF3- and Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	72.020, 51.490, 88.020
Unit cell angles	90.00, 110.82, 90.00

Refinement procedure

Resolution	41.140 - 1.750
R-factor	0.1717
Rwork	0.170
R-free	0.21130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rvn
RMSD bond length	0.030
RMSD bond angle	3.237
Data reduction software	XDS (1.18.2_3874)
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.140	1.850
High resolution limit [Å]	1.750	1.750
Number of reflections	30098	2991
<I/σ(I)>	8.15
Completeness [%]	98.4
Redundancy	4.7
CC(1/2)	0.997	0.334

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris-HCl pH 8.8, 25% PEG 5000 MME