7OG1

AP2 clathrin adaptor core in complex with cargo peptide and FCHO2

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.92001
Spacegroup name	P 1 21 1
Unit cell lengths	92.576, 150.070, 96.430
Unit cell angles	90.00, 112.65, 90.00

Refinement procedure

Resolution	76.662 - 3.250
Rwork	0.207
R-free	0.30630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xa7
RMSD bond length	0.006
RMSD bond angle	1.578
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.580	3.390
High resolution limit [Å]	3.250	3.250
Rmerge	0.133	2.123
Rmeas	0.159	2.590
Rpim	0.086	1.465
Number of reflections	38355	4690
<I/σ(I)>	7.2	0.8
Completeness [%]	100.0	100
Redundancy	6.6	6
CC(1/2)	0.998	0.366

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	Crystals of AP-2 in complex with FCHO2 linker and TGN38 peptide, supplemented with 10mM K Na Tartrate, grew in sitting drops with reservoir 0.1M Mg formate dehydrate, 10% to 15% PEG 3350. The crystals were cryo-protected with 0.1M Mg formate dehydrate, 13% PEG 3350, 18-24% Glycerol and 1mg/ml of peptide.