7OFP

Apo Structure of Mu2 Adaptin Subunit (Ap50) Of AP2 Clathrin Adaptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 65
Unit cell lengths	123.830, 123.830, 112.668
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	77.680 - 1.920
R-factor	0.1892
Rwork	0.188
R-free	0.21050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bxx
RMSD bond length	0.018
RMSD bond angle	1.254
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (1.19rc1_4016)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.680	1.950
High resolution limit [Å]	1.920	1.920
Rmerge	0.223	6.379
Rmeas	0.230	6.664
Rpim	0.055	1.912
Number of reflections	74711	3642
<I/σ(I)>	13.1	1
Completeness [%]	99.9	98
Redundancy	17.7	12.1
CC(1/2)	0.999	0.440

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	Protein (10 mg/ml) and peptide (DVDEEGYSIKPETNQNDTKENHFYSS) (2mg/ml) were equilibrated against 1.5M Ammonium Sulphate 0.1M Hepes pH 7.0. Crystals were cryo-protected by soaking in mother liquor supplemented with 20% glycerol and peptide (peptide did not crystallise).