7OEZ

Leucine Aminopeptidase A mature enzyme in a complex with leucine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97950
Spacegroup name	P 63 2 2
Unit cell lengths	154.100, 154.100, 87.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	77.050 - 2.480
R-factor	0.1595
Rwork	0.157
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rtq
Data reduction software	APEX
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.050	2.569
High resolution limit [Å]	2.480	2.480
Rmerge	0.097	0.483
Number of reflections	22302	2195
<I/σ(I)>	7.08
Completeness [%]	100.0
Redundancy	2
CC(1/2)	0.982	0.556

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M Citrate, pH =5.0, 3.2M Ammonium Sulphate