7ODZ
Dictyostelium discoideum dye decolorizing peroxidase DyPA in complex with an activated form of oxygen and veratryl alcohol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.910003 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 140.440, 140.440, 95.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.110 - 1.600 |
| R-factor | 0.1517 |
| Rwork | 0.151 |
| R-free | 0.16820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7o9j |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.640 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.022 | 0.279 |
| Number of reflections | 125165 | 20558 |
| <I/σ(I)> | 25.13 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 33.26 | 33.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 2.4 M Sodium malonate pH 7.0 |
