7OCX

Crystal Structure of the PID-3 TOFU-6 RRM domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.00, 1.54
Spacegroup name	P 21 21 21
Unit cell lengths	43.980, 68.002, 130.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.680 - 1.700
R-factor	0.1958
Rwork	0.194
R-free	0.23170
Structure solution method	SIRAS
RMSD bond length	0.009
RMSD bond angle	1.092
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.680	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.086
Rpim	0.025
Number of reflections	43394	3808
<I/σ(I)>	16.24	0.56
Completeness [%]	97.9
Redundancy	12.7
CC(1/2)	0.999	0.171

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M Sodium acetate pH 5.0 0.2 M Sodium chloride 17 % PEG 3350