7OCL

K1K1, a potent recombinant minimal hepatocyte growth factor/scatter factor mimic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.953725
Spacegroup name	P 1 21 1
Unit cell lengths	45.570, 58.750, 65.940
Unit cell angles	90.00, 108.22, 90.00

Refinement procedure

Resolution	43.290 - 1.800
R-factor	0.1981
Rwork	0.197
R-free	0.22710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Kringle 1
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.290	43.290	1.670
High resolution limit [Å]	1.610	6.240	1.610
Rmerge	0.154	0.080	2.144
Rmeas	0.172	0.090	2.392
Rpim	0.074	0.040	1.048
Total number of observations	224081	3805	21599
Number of reflections	42914	781	4179
<I/σ(I)>	4.6	11.9	0.6
Completeness [%]	99.9	99.3	99.8
Redundancy	5.2	4.9	5.2
CC(1/2)	0.986	0.928	0.454

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	290.15	100 mM Tris/Bicine pH 8.5, 30 mM Sodium nitrate, 30 mM Sodium phosphate dibasic, 30 mM Ammonium sulfate, 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD