7OBJ
Carbon regulatory PII-like protein SbtB from Synechocystis sp. 6803 in complex with cyclic di-AMP (c-di-AMP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.000 |
Spacegroup name | P 32 |
Unit cell lengths | 63.171, 63.171, 81.733 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.740 - 2.000 |
R-factor | 0.1797 |
Rwork | 0.178 |
R-free | 0.21500 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5o3r |
RMSD bond length | 0.017 |
RMSD bond angle | 1.927 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.740 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.064 | 2.231 |
Number of reflections | 24685 | 3942 |
<I/σ(I)> | 20.61 | 1.03 |
Completeness [%] | 99.7 | |
Redundancy | 10.3 | 10.1 |
CC(1/2) | 1.000 | 0.430 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1M phosphate-citrate pH 4.2, 40% (w/v) PEG 300 |