7OB3

hSTING in complex with 3',3'-c-di-araAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-18
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	67.335, 67.335, 134.945
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.000 - 2.300
R-factor	0.215
Rwork	0.214
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ksy
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	2.382
High resolution limit [Å]	2.092	2.300
Number of reflections	26101	1404
<I/σ(I)>	10.27
Completeness [%]	98.7
Redundancy	12.3
CC(1/2)	0.996	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	291	0.2 M Sodium chlorid, 0.1 M Tris pH 7.4, 30% PEG 300, 10 mM EDTA