7OB0
Structure of RsLOV d2 variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS MICROFOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-11-27 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.057, 77.168, 155.002 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.930 - 1.900 |
| R-factor | 0.1776 |
| Rwork | 0.176 |
| R-free | 0.21810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hia |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.918 |
| Data reduction software | XDS |
| Data scaling software | pointless (1.12.2) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.057 | 0.254 |
| Number of reflections | 55587 | 8900 |
| <I/σ(I)> | 17.67 | 5.53 |
| Completeness [%] | 99.6 | |
| Redundancy | 3.93 | |
| CC(1/2) | 0.998 | 0.961 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 0.1 M Imidazole pH 8.0, 22 %(w/v) PEG 1000, 0,2 M Calcium acetate |
