7OA8

PilC minor pilin of Streptococcus sanguinis SK36 type IV pili

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	38.640, 96.030, 109.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.010 - 1.600
Rwork	0.209
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7OA7.pdb
RMSD bond length	0.005
RMSD bond angle	0.732
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.010	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	54911	2647
<I/σ(I)>	17.2
Completeness [%]	100.0
Redundancy	6.4
CC(1/2)	0.999	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M magnesium chloride hexahydrate; 20 % w/v polyethylene glycol 3350