7OA7
PilC minor pilin of Streptococcus sanguinis 2908 type IV pili
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-14 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 96.481, 96.481, 85.078 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 83.560 - 1.450 |
Rwork | 0.183 |
R-free | 0.20100 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.000 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | autoSHARP |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.080 | 1.470 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 81269 | 4043 |
<I/σ(I)> | 15.4 | |
Completeness [%] | 100.0 | |
Redundancy | 19.97 | |
CC(1/2) | 0.999 | 0.541 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium sulphate; 0.01 M cadmium chloride hemi (pentahydrate); 0.1 M Pipes (pH 7.0); 15 % v/v PEG smear broad; 10 % v/v ethylene glycol |