7O9Z

Crystal structure of Human Menin in complex with BD-08

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 43 2 2
Unit cell lengths	107.193, 107.193, 107.443
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.028 - 1.979
R-factor	0.2129
Rwork	0.211
R-free	0.24350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ddc
RMSD bond length	0.010
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.030	2.050
High resolution limit [Å]	1.979	1.979
Rmerge	0.076	0.515
Rmeas	0.080	0.538
Number of reflections	44239	4358
<I/σ(I)>	19.08	3.62
Completeness [%]	99.9	99.73
Redundancy	11.7	11.5
CC(1/2)	0.998	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	12% PEG 3350 14% Glycerol